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Registros recuperados: 79
Primeira ... 1234 ... Última
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Detection of poliovirus type 2 in oysters by using cell culture and RT-PCR BJM
Vinatea,Cecília E.B.; Sincero,Thaís C.M.; Simões,Claúdia M.O.; Barardi,Célia R.M..
Shellfish are readily contaminated with viruses present in water containing sewage due to the concentrating effect of filter feeding. Enteroviruses are generally used as a model for the detection of viruses from shellfish due to their public health significance. In the present work, oysters were placed in glass aquaria containing seawater plus unicellular algae. Two experiments were performed: 1) oysters bioaccumulating four different poliovirus type 2 concentrations: 5 x 10(4), 2.5 x 10(4), 5 x 10³ and 5 x 10² PFU/mL during 20h; 2) oyster tissues directly inoculated with 6.0 x 10(5) and 1.0 x 10(5) PFU/mL. After viruses seeding, tissue samples were processed by an adsorption-elution-precipitation method. Positive controls were performed by seeding 6.0 x...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oysters; Poliovirus; Bioaccumulation; Cell culture; RT-PCR.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100012
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Development of a flow-through system for the study of toxicity and bioaccumulation processes in aquatic invertebrates OMA
Blust, R.; Decleir, W..
A flow-through test system for the study of toxicity and bioaccumulation processes is described. The apparatus can operate within a very wide range of flow rates. Flow deviations are less than 2% and the variation in test-agent concentration, (determined for copper) ranges from 3 to 8%. As a practical test a copper-toxicity experiment on adult <i>Artemia</i> was carried out and the results compared with data obtained from a similar but static assay. The results indicate that: - bioassay and accumulation experiments must preferably be carried out under flow-through conditions to keep the concentration and bioavailability of the toxicant as constant as possible;- the data analysis must be based upon measured test-solution toxicant-concentrations...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioaccumulation; Marine invertebrates; Toxicity tests.
Ano: 1984 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3261
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Dietary exposure of juvenile common sole (Solea solea L.) to polybrominated diphenyl ethers (PBDEs): Part 1. Bioaccumulation and elimination kinetics of individual congeners and their debrominated metabolites ArchiMer
Munschy, Catherine; Moisan, Karine; Tixier, Celine; Olivier, Nathalie; Gastineau, Olivier; Le Bayon, Nicolas; Buchet, Vincent.
The uptake and elimination of six PBDE congeners (BDE-28, -47, -99, -100, -153, -209) were studied in juvenile common sole (Solea solea L) exposed to spiked contaminated food over a three-month period, then depurated over a five-month period. The results show that all of the studied PBDEs accumulate in fish tissues, including the higher brominated congener BDE-209. Several additional PBDE congeners were identified in the tissues of exposed fish, revealing PBDE transformation, mainly via debromination. The identified congeners originating from PBDE debromination include BDE-49 and BDE-202 and a series of unidentified tetra-, penta-, and hepta- BDEs. Contaminant assimilation efficiencies (AEs) were related to their hydrophobicity (log K-ow) and influenced by...
Tipo: Text Palavras-chave: Marine flatfish; PBDEs; Bioaccumulation; Biotransformation; Debromination.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00021/13188/11296.pdf
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Dietary exposure of juvenile common sole (Solea solea L.) to polybrominated diphenyl ethers (PBDEs): Part 2. Formation, bioaccumulation and elimination of hydroxylated metabolites ArchiMer
Munschy, Catherine; Moisan, Karine; Tixier, Celine; Pacepavicius, G.; Alaee, M..
The uptake, elimination and transformation of six PBDE congeners (BDE-28, -47, -99, -100, -153, -209) were studied in juvenile common sole (Solea solea L) exposed to spiked contaminated food over a three-month period, and then depurated over a five-month period. Methoxylated (MeO-) and hydroxylated (OH-) PBDEs were determined in fish plasma exposed to PBDEs and compared to those obtained in control fish. While all MeO- and some OH- congeners identified in fish plasma were found to originate from non-metabolic sources, several OH- congeners, i.e., OH-tetraBDEs and OH-pentaBDEs, were found to originate from fish metabolism. Among these, 4 '-OH-BDE-49 was identified as a BDE-47 metabolite. Congener 4 '-OH-BDE-101, identified here for the first time, may be...
Tipo: Text Palavras-chave: Marine flatfish; PBDEs; Bioaccumulation; Biotransformation; Hydroxylated metabolites.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00017/12811/11295.pdf
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Distribution and relationships of heavy metals in the giant clam (Tridacna maxima) and associated sediments from different sites in the Egyptian Red Sea Coast OceanDocs
Madkour, H.A..
The giant clam (Tridacna maxima) and sediments have been collected from clean and contaminated coastal sites of the Egyptian Red Sea. Selected samples of the giant clam shells and the associated surface sediments were analyzed for Fe, Mn, Zn, Cu, Pb, Ni and Cd. Significant spatial differences in metal concentrations in Tridacna maxima and sediments were identified. Copper and lead are greatly enriched in the giant clam shells, which is related to their physiological function. Cd content is higher in Tridacna maxima than in sediments, because of the easy substitution between Cd and Ca. The levels of most metals in the giant clam shells and sediments were higher in the anthropogenic sites than in the uncontaminated sites. Generally, metal variations reflect...
Tipo: Journal Contribution Palavras-chave: Bioaccumulation; Heavy metals; Giant clam; Tridacna; Red Sea Accumulation Chemical analysis Clam culture Absorption http://aims.fao.org/aos/agrovoc/c_27.
Ano: 2005 URL: http://hdl.handle.net/1834/1307
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Diversidad de peces demersales en la plataforma continental del Ecuador OceanDocs
Moncayo, D.; Trejos, R.; Maridueña, A.; Castro, R..
Se determine el contenido de Mercurio (Hg), Cadmio (Cd) y Plomo (Pb) en 18363 muestras de productos pesqueros colectadas durante el período enero del 2006 hasta julio del 2009. La determinación fue realizada empleando espectrofotometría de Absorción Atómica por generación de vapor frío (Hg) y horno de grafito (Cd y Pb), todas las concentraciones son expresadas en peso húmedo. Los análisis fueron realizados con el propósito de verificar que los productos pesqueros a ser exportados cumplen con los niveles establecidos en el Reglamento consolidado (CE)N°1881/2006, de la Unión Europea, que fija el conte¬nido máximo de determinados contaminantes en los productos alimenticios. Las concentraciones encontradas, en las muestras de peces, en su mayoría fueron...
Tipo: Journal Contribution Palavras-chave: Heavy metals; Atomic absorption spectroscopy; Bioaccumulation; Pollution effects; Fishery products.
Ano: 2010 URL: http://hdl.handle.net/1834/4802
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Editorial: New Challenges in Marine Pollution Monitoring ArchiMer
Bellas, Juan; Hylland, Ketil; Burgeot, Thierry.
Tipo: Text Palavras-chave: Marine pollution; Ecotoxicology; Biomonitoring; Water quality; Bioaccumulation.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00606/71775/70269.pdf
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Effets de pollutions par hydrocarbures sur les capacités de défense d'organismes marins ArchiMer
Bado Nilles, Anne.
The effects of PAHs, considered among the most toxic by the United States Environmental Protection Agency, were tested in vitro and in vivo on two commercial species of the Pertuis-Charentais (Charente-Maritime, France): sea bass, Dicentrarchus labrax, and the Pacific oyster, Crassostrea gigas. This study was carried out as part of the European project EROCIPS with the aim of finding new immunological biomarkers caused by occasional pollution by hydrocarbons. During in vitro experimentation, pollutants and immunological biomarkers were choosen. Thereafter, the in vitro exposures to the soluble fraction of Erika's heavy fuel oil and its fluxant, light cycle oil, began. These exposures enable the validation of the experimental system used, with, in...
Tipo: Text Palavras-chave: Biliary metabolites; Bioaccumulation; Toxicity; Immune défense; European sea bass; Pacific oyster; Métabolites biliaires; Bioaccumulation; Toxicité; Capacités de défense; Hydrocarbure; Bar commun; Huître creuse.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/these-7401.pdf
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Efflorescences toxiques des eaux côtières françaises. Ecologie, écophysiologie, toxicologie ArchiMer
Berland, Brigitte; Lassus, Patrick.
Tandis que certa ines efflorescences micro-algales provoquent de sérieuses nuisances par leur développement exubérant, d'autres sont nocives en raison des toxines qu'elles produisent. Ces proliférations,  dangereuses pour les organismes marins, mais aussi pour les consommateurs de fruits de mer, sont étudiées en France depuis 1983. Dès 1989, devant l'ampleur des phénomènes observés au niveau national et européen, un programme scientifique réunissant de nombreux in stituts et laboratoires, le PNEAT, a été créé afin de fédérer les moyens mis en oeuvre dans des disci plines aussi diverses que l'écologie, la biologie cellulaire, l'écophysiologie, la toxicologie et la chimie. Les résultats obtenus au cours de la période 1989-1995 sont présentés à la fois pour...
Tipo: Text Palavras-chave: Efflorescences; Cycle biologique; Allélopathie; Taxonomie; Ecophysiologie; Broutage; Phycotoxines; Toxicologie; Chimie extractive; Toxinogenèse; Bioaccumulation.
Ano: 1997 URL: https://archimer.ifremer.fr/doc/00450/56179/57726.pdf
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Etude quantitative et qualitative des métallothioneines chez la daurade "Sparus aurata" exposée aux métaux lourds OceanDocs
Ameur, S.; Ghedira, J.; Guerbej, H.; Jebali, J.; Bouraoui, J.; Boussetta, H..
Dans l’environnement marin, les éléments traces peuvent s’accumuler dans les organismes à des concentrations supérieures à celles présentes dans l’eau. L’étude de la distribution tissulaire des métaux traces au niveau des organismes qui y sont exposés, constitue un aspect très important pour la compréhension de leur devenir. En outre, le suivi de la réponse biologique spécifique de ces organismes sentinelles aux xénobiotiques complète et consolide l’analyse chimique. Ce travail vise à étudier la distribution tissulaire des métaux traces et des métallothionéines (MTs) chez la daurade «Sparus aurata » traitée par un métal essentiel (le cuivre) et un métal non essentiel (le cadmium) à une concentration de 500µg /Kg de poids frais pendant 48 heures....
Tipo: Journal Contribution Palavras-chave: Animal organs; Bioaccumulation; Cadmium; Gills; Heavy metals; Metals; Pollutants; Pollution effects; Sediment pollution; Toxicity tests; Trace metals; Marine sciences; Biomarkers of exposure (organisms); Metallic elements.
Ano: 2009 URL: http://hdl.handle.net/1834/4448
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Etude saisonnière de la contamination métallique des macroalgues de la lagune nord de Tunis OceanDocs
Chouba, L.; Ajjabi-Chebil, L.; Herry, S..
Dans le but de démontrer la capacité de bioaccumulation métallique des macroalgues et sa variation temporelle, on s’est intéressé à la détermination de la gamme de concentrations en métaux traces (Plomb, Cadmium, Fer, Manganèse et Zinc) dans trois macroalgues vertes (Ulva rigida, Chaetomorpha linum, Caulerpa prolifera) au niveau de la lagune nord et du canal de navigation de Tunis sujettes à d’importants rejets anthropiques. Des collectes saisonnières ont été faites dans la lagune de Tunis durant la période d’avril 2009 au février 2010. Les teneurs métalliques déterminées par spectrophotométrie d’absorption atomique ont montré des variations significatives selon l’espèce et la saison de prélèvement. Les résultats ont montré que les teneurs en différents...
Tipo: Journal Contribution Palavras-chave: Heavy metals; Algae; Seaweeds; Bioaccumulation; Green algae.
Ano: 2010 URL: http://hdl.handle.net/1834/4733
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Evaluación de la tasa fotosintética del alga verde Rhizoclonium riparium (Roth) Kützing ex Harvey, expuesta a cobre y su uso como indicador de contaminación por metales en aguas estuarinas OceanDocs
Montoya, W.; Peña, E.J.; Benítez, R..
Algae have been used as bioindicators by having several advantages such as they have short life cycles, they can take up nutrients directly from water, they are generally easy to collect in sufficient amounts from various habitats; and they readily accumulate compounds present in seawater, making tissue analyses reliable indicators of water quality. In order to evaluate the bioremoval capability of the green alga Rhizoclonium riparium, the photosynthetic responses to heavy metal pollution under different sublethal concentrations of copper was assessed. Photosynthetic rates were significantly low (p <0.05) at low copper concentrations, between (0.01 y 10 mg/l). Maximum photosynthetic values were shown at 30 y 60 mg/l and almost cero at 150 mg/ l of Cu....
Tipo: Conference Material Palavras-chave: Heavy metals; Biological stress; Algae; Indicator species; Pollution; Bioaccumulation; Copper; Water quality; Estuaries; Heavy metals; Pollution; Bioaccumulation; Copper; Water quality; Estuaries; Algae; Indicator species; Http://aims.fao.org/aos/agrovoc/c_2223; Http://aims.fao.org/aos/agrovoc/c_6077; Http://aims.fao.org/aos/agrovoc/c_32389; Http://aims.fao.org/aos/agrovoc/c_1868; Http://aims.fao.org/aos/agrovoc/c_16061; Http://aims.fao.org/aos/agrovoc/c_2673; Http://aims.fao.org/aos/agrovoc/c_258; Http://aims.fao.org/aos/agrovoc/c_35790.
Ano: 2006 URL: http://hdl.handle.net/1834/2256
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Evaluation de la contamination chimique par les métaux traces (Cd, Pb, Hg et Zn) du zooplancton et de la sardinelle <Sardinella aurita>dans le golfe de tunis OceanDocs
Ennouri, R.; Chouba, L.; Kraeim, M.M..
La présente étude a pour but de déterminer l’organotropisme de quatre métaux traces (Cd, Pb, Hg et Zn) ainsi que leurs concentrations dans le zooplancton et dans trois organes (muscle, foie et branchies) de la sardinelle (Sardinella aurita) provenant du golfe de Tunis. Les résultats ont montré que les concentrations moyennes des quatre métaux dosés (Cd, Pb, Hg et Zn) sont plus élevées dans la sardinelle que dans le zooplancton. Ceci est dû à la bioamplification de ces éléments dans la sardinelle qui occupe un niveau trophique élevé de la chaîne alimentaire marine. Pour le Pb, le Hg et le Zn, les teneurs le plus élevées ont été enregistrées dans les branchies de la sardinelle, alors que le Cd est concentré davantage au niveau du foie. Les concentrations...
Tipo: Journal Contribution Palavras-chave: Bioaccumulation; Marine pollution; Pollution indicators; Trace elements; Zooplankton; Sardinella aurita; Bioaccumulation; Zooplankton; Sardinella aurita; Trace elements; Http://aims.fao.org/aos/agrovoc/c_32389; Http://aims.fao.org/aos/agrovoc/c_15490; Http://aims.fao.org/aos/agrovoc/c_39038; Http://aims.fao.org/aos/agrovoc/c_7834.
Ano: 2008 URL: http://hdl.handle.net/1834/3707
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Evaluation de la contamination chimique par les metaux traces (cd, pb, hg et zn) du zooplancton et de la sardinelle (Sardinella aurita) dans le golfe de tunis OceanDocs
Chouba, L.; Ennouri, R.; Kraiem, M.M..
La présente étude a pour but de déterminer l’organotropisme de quatre métaux traces (Cd, Pb, Hg et Zn) ainsi que leurs concentrations dans le zooplancton et dans trois organes (muscle, foie et branchies) de la sardinelle (Sardinella aurita) provenant du golfe de Tunis. Les résultats ont montré que les concentrations moyennes des quatre métaux dosés (Cd, Pb, Hg et Zn) sont plus élevées dans la sardinelle que dans le zooplancton. Ceci est dû à la bioamplification de ces éléments dans la sardinelle qui occupe un niveau trophique élevé de la chaîne alimentaire marine. Pour le Pb, le Hg et le Zn, les teneurs le plus élevées ont été enregistrées dans les branchies de la sardinelle, alors que le Cd est concentré davantage au niveau du foie. Les concentrations...
Tipo: Journal Contribution Palavras-chave: Bioaccumulation; Marine pollution; Pollution indicators; Trace elements; Zooplankton; Sardinella aurita; Marine environment; Http://aims.fao.org/aos/agrovoc/c_39038; Http://aims.fao.org/aos/agrovoc/c_4610.
Ano: 2008 URL: http://hdl.handle.net/1834/4155
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Experimental evidence of dietary ciguatoxin accumulation in an herbivorous coral reef fish ArchiMer
Clausing, Rachel J.; Losen, Barbara; Oberhaensli, Francois R.; Darius, H. Taiana; Sibat, Manoella; Hess, Philipp; Swarzenski, Peter W.; Chinain, Mireille; Bottein, Marie-yasmine Dechraoui.
Ciguatoxins (CTXs) are potent algal toxins that cause widespread ciguatera poisoning and are found ubiquitously in coral reef food webs. Here we developed an environmentally-relevant, experimental model of CTX trophic transfer involving dietary exposure of herbivorous fish to the CTX-producing microalgae Gambierdiscus polynesiensis. Juvenile Naso brevirostris were fed a gel-food embedded with microalgae for 16 weeks (89 cells g.1 fish daily, 0.4 ¦Ìg CTX3C equiv kg.1 fish). CTXs in muscle tissue were detectable after 2 weeks at levels above the threshold for human intoxication (1.2 ¡À 0.2 ¦Ìg CTX3C equiv kg.1). Although tissue CTX concentrations stabilized after 8 weeks (¡«3 ¡À 0.5 ¦Ìg CTX3C equiv kg.1), muscle toxin burden (total ¦Ìg CTX in muscle tissue)...
Tipo: Text Palavras-chave: Ciguatoxin; Bioaccumulation; Growth dilution; Trophic transfer; Herbivorous fish; Experimental model.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00442/55339/60476.pdf
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Fate of Cerium Oxide Nanoparticles in Natural Waters and Immunotoxicity in Exposed Rainbow Trout ArchiMer
Gagnon, C.; Bruneau, Audrey; Turcotte, P.; Pilote, M.; Gagne, F..
Once released in the environment, engineered nanoparticles (NPs) can undergo important transformation resulting in changed properties under natural conditions. This study investigated the fate, the bioavailability and the immunotoxicity of cerium oxide (CeO2) nanoparticles in fish exposed to CeO2 in representative surface waters differing in pH, organic matter content and conductivity (green and brown waters). Following an incubation period of NP CeO2 in different surface waters, particle size distribution and shape were determined by ultrafiltration and ICP-mass spectrometry, electronic microscopy and dynamic light scattering (DSL). Bioaccumulation and effect biomarkers focusing on the immune system responses (viability of immune cells and phagocytic...
Tipo: Text Palavras-chave: Nanoparticle; Nanotoxicity; Bioaccumulation; Transformation.
Ano: 2018 URL: http://archimer.ifremer.fr/doc/00433/54422/55765.pdf
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Geographic, seasonal and ontogenetic variation in cadmium and mercury concentrations in squid (Cephalopoda : Teuthoidea) from UK waters ArchiMer
Pierce, G; Stowasser, G; Hastie, L; Bustamante, Paco.
Cadmium (Cd) and mercury (Hg) levels were measured in the tissue samples of two loliginid (Alloteuthis sp. and Loligo forbesi) and two ommastrephid (Todarodes sagittatus and Todaropsis eblanae) squid species collected from research cruise and fishery (market) samples in UK waters during 2004-05. Concentrations of Cd were generally higher in the ommastrephids, in all tissues except muscle. Hg concentrations were higher in T sagittatus than in the loliginids. In L. forbesi, metal concentrations differed between tissues and also varied in relation to body size, geographic origin, and season. Cd levels decreased with increasing body size. This may be related to a shift in the diet with growth, since small L. forbesi feed on benthic invertebrates that have...
Tipo: Text Palavras-chave: Risk for human consumption; Bioaccumulation; Trace elements; Heavy metals; Cephalopods.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4356.pdf
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Growth and metal uptake of microalgae produced using salt groundwaters from the Bay of Bourgneuf ArchiMer
Gagneux Moreaux, Sindy; Cosson, Richard P.; Bustamante, Paco; Moreau, Christophe.
The Bay of Bourgneuf, France, is a main site of shellfish production. In the marshes along the bay, oyster intensive rearing and fattening need the mass production of microalgae. Salt groundwaters, available in this region, support a large part of this production for aquaculture. Studies carried out by local authorities have brought to the fore the accumulation of lead (Pb) in several samples of algal pastes derived from cultures using salt groundwater. The aim of this study was to compare growth, nutritional value and metal (Cd, Cu, Pb and Zn) uptake of four microalgae grown in two salt groundwaters or in enriched coastal seawater. Cultures of microalgae used in aquaculture (Haslea ostrearia, Phaeodactylum tricornutum, Skeletonema costatum and Tetraselmis...
Tipo: Text Palavras-chave: Marine microalgae cultivation; Aquaculture; Metals; Growth; Prasinophycea; Bacillariophycea; Diatom; Bioaccumulation; Trace elements; Saline groundwater.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2135.pdf
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Histological Description of the Skin Glands of Phyllobates bicolor (Anura: Dendrobatidae) Using Three Staining Techniques International Journal of Morphology
Moreno-Gómez,Freddy; Duque,Tania; Fierro,Leonardo; Arango,Juan; Peckham,Xiomara; Asencio-Santofimio,Helberg.
The mechanisms to obtain and store skin toxins in frogs in of the family Dendrobatidae are not completely understood. In order to contribute to understand how toxins are stored, we provide a histological description of the cutaneous glands of the species Phyllobates bicolor. The skin of two adult frogs was examined through three histological staining techniques (hematoxilin-eosin, PAS and Masson Trichrome) using conventional optic microscopy. The skin of Phyllobates bicolor contains two types of exocrine glands: mucous and serous, which empty their products to the epidermal surface through an intra-epithelial duct that leads to a stoma. The mucous and serous glands and the intercalated ducts are surrounded by a discontinuous sheath of myoepithelial cells,...
Tipo: Journal article Palavras-chave: Amphibians; Phyllobates bicolor; Integument; Epidermis; Dermis; Mucous glands; Serous glands; Chemical defenses; Bioaccumulation.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000300022
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Impact of Anthropogenic Activities on Water Quality of The Paranhana River, Southern Brazil BABT
Dalzochio,Thaís; Souza,Mateus Santos de; Simões,Leonardo Airton Ressel; Silva,Gláucia Joselaine Herbert; Rodrigues,Gabriela Zimmermann Prado; Andriguetti,Natália Bordin; Silva,Luciano Basso da; Gehlen,Günther.
Abstract: The Paranhana River, located in Southern Brazil, is one of the major tributaries of the Sinos River basin and receives mainly industrial and domestic effluents. In the present study, water physicochemical and microbiological analyses, condition factor, micronucleus test, gill histopathology and metal bioaccumulation in the muscle of the native fish Bryconamericus iheringii collected at two sites (S1 and S2) of the Paranhana River under different degrees of anthropogenic pressures were assessed in four sampling campaigns. Data from water quality parameters, condition factor, mucous cells proliferation in fish gills and bioaccumulation of chromium and manganese in muscle evidenced higher impacts at S2, whereas a higher genotoxic potential was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biomarkers; Paranhana River; Bioaccumulation; Histopathology; Micronucleus.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100421
Registros recuperados: 79
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